Browsing by Author "Briz, N."
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Item Compact and cost effective instrument for detecting drug precursors in different environments based on fluorescence polarization(SPIE-INT SOC OPTICAL ENGINEERING, 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98227-0010 USA, 2013) Antolín-Urbaneja, Juan Carlos; Eguizabal, I.; Briz, N.; Dominguez, A.; Estensoro, Patxi; Secchi, A.; Varriale, A.; Di Giovanni, S.; D´Auria, S.; Tecnalia Research & Innovation; ROBOTICA_AUTOMA; TECNOLOGÍAS DE HIDRÓGENO; SG; MAQUINAS; GENERALSeveral techniques for detecting chemical drug precursors have been developed in the last decade. Most of them are able to identify molecules at very low concentration under lab conditions. Other commercial devices are able to detect a fixed number and type of target substances based on a single detection technique providing an absence of flexibility with respect to target compounds. The construction of compact and easy to use detection systems providing screening for a large number of compounds being able to discriminate them with low false alarm rate and high probability of detection is still an open concern. Under CUSTOM project, funded by the European Commission within the FP7, a stand-alone portable sensing device based on multiple techniques is being developed. One of these techniques is based on the LED induced fluorescence polarization to detect Ephedrine and Benzyl Methyl Keton (BMK) as a first approach. This technique is highly selective with respect to the target compounds due to the generation of properly engineered fluorescent proteins which are able to bind the target analytes, as it happens in an “immune-type reaction”. This paper deals with the advances in the design, construction and validation of the LED induced fluorescence sensor to detect BMK analytes. This sensor includes an analysis module based on high performance LED and PMT detector, a fluidic system to dose suitable quantities of reagents and some printed circuit boards, all of them fixed in a small structure (167mm x 193mm x 228mm) with the capability of working as a stand-alone application.Item Compact and cost effective instrument for detecting drug precursors in different environments based on fluorescence polarization (Poster)(2013) Antolín-Urbaneja, Juan Carlos; Eguizabal, I.; Briz, N.; Dominguez, A.; Estensoro, Patxi; Secchi, A.; Varriale, A.; Di Giovanni, S.; D´Auria, S.; Tecnalia Research & InnovationSeveral techniques for detecting chemical drug precursors have been developed in the last decade. Most of them are able to identify molecules at very low concentration under lab conditions. Other commercial devices are able to detect a fixed number and type of target substances based on a single detection technique providing an absence of flexibility with respect to target compounds. The construction of compact and easy to use detection systems providing screening for a large number of compounds being able to discriminate them with low false alarm rate and high probability of detection is still an open concern. Under CUSTOM project, funded by the European Commission within the FP7, a stand-alone portable sensing device based on multiple techniques is being developed. One of these techniques is based on the LED induced fluorescence polarization to detect Ephedrine and Benzyl Methyl Keton (BMK) as a first approach. This technique is highly selective with respect to the target compounds due to the generation of properly engineered fluorescent proteins which are able to bind the target analytes, as it happens in an “immune-type reaction”. This paper deals with the advances in the design, construction and validation of the LED induced fluorescence sensor to detect BMK analytes. This sensor includes an analysis module based on high performance LED and PMT detector, a fluidic system to dose suitable quantities of reagents and some printed circuit boards, all of them fixed in a small structure (167mm x 193mm x 228mm) with the capability of working as a stand-alone application.Item Effects of ion implantation on nano-topographic properties(2007-08-05) Braceras, I.; Briz, N.; García, F.; Muñoz, R.; Viviente, J.L.; Onate, J.I.; INGENIERÍA DE SUPERFICIES; SG; TECNOLOGÍA DE MEMBRANAS E INTENSIFICACIÓN DE PROCESOS; Tecnalia Research & InnovationIt is well known that surface properties at nano-scale are determinant in a number of applications, such as sensors, biomedical and optical devices. Nevertheless, relations between surface treatment parameters and their effects on topography at the nano-scale, surface energy or light reflectivity are often poorly understood. In this study, a non fluorescent glass material (Knittel) was selected and subjected to ion implantation treatments with different parameters and species, including Ar, Ne, C, N, CO and NH2. The resulting surface topography at the nano-scale was studied by Atomic Force Microscopy (AFM) and the surface energy was evaluated with the contact angle method. Additionally, the modifications induced on optical properties, i.e. reflection, were evaluated with two different wavelength lasers. The results showed remarkable differences in surface nano-topographies and contact angles (from 15° to 70°) that were obtained. Furthermore, the effects of ion implantation parameters had also very significant consequences on background noise effects, of great importance for optical properties. It was found that the best implantation treatment corresponded to N2 + ions implanted to a dose of 3×1017 ions/cm2 at an energy of 30 keV. This treatment resulted in a adequate contact angle, producing a nano-textured surface with potential features for a good attachment and orientation of deposited bio-molecules, and a very low background fluorescence, hence allowing a high degree of scanning sensitivity, for application on DNA microarrays. The study has shown that ion implantation represents a powerful tool for modifying key properties on surfaces that play an important role in the response elicited on living tissue and bio-molecules, which is notoriously relevant for the application as bio-sensors.Item Fabrication and Characterization of a Novel Bioactive Poly(ether-ether-ketone) Scaffold for Bone Tissue Engineering (Poster)(MARY ANN LIEBERT, INC, 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA, 2015-09) Olalde, Beatriz; Oyarbide, Joseba; Ayerdi, Ana; Azpiroz, Patxi; Fernandez, Ruben; Aizpurua, J.; Braceras, I.; Alvarez, Noelia; Briz, N.; Morin, F.O.It is widely accepted that the initial interactions between the cells and implant surface are crucial to clinical success. One of the promising alternative materials for tissue engineering is polyetheretherketone (PEEK) which has good chemical resistance and mechanical properties similar to those of human bones [1]. However, its hydrophobic and chemically inert surface limits local bone attachment [2]. Consequently, there have been efforts to enhance PEEK bone-implant integration by incorporating molecules (such as HA) or making it porous [3]. Regarding to the latest, this study aims to demonstrate a process that can greatly enhance the density and interconnectivity of a PEEK porous product (US2012323339) [4]. Moreover, a novel surface modification through wet-chemistry protocol has been developed for RGD and OGP (osteogenic growth peptide) grafting onto porous PEEK scaffolds (US2013172273) [5]. As a result, the production of porous structures manufactured by thermally induced phase separation technique (TIPS), in combination with particulate leaching method, offers the greatest potential to control pore size and porosity whilst achieving porosity higher than 80%. Besides, surface functionalization of porous PEEK scaffolds with RGD/OGP combination is shown to enhance osteoblast adhesion, proliferation and differentiation, according to the in vitro results. In conclusion, we have developed a convenient “click” chemistry procedure to modify PEEK surfaces with RGD and OGP10-14 molecules, while keeping the bulk properties of PEEK as well as its biocompatibility in terms of cytotoxicity. The improvement of the osteogenic activity of this novel material shows that can be appropriated for bone tissue engineering applications.