Browsing by Author "Barroca, Nathalie"
Now showing 1 - 2 of 2
Results Per Page
Sort Options
Item Circuit Modeling of rGO-doped Scaffolds for Spinal Cord Regeneration Based on Transient and xAC Analyses(Institute of Electrical and Electronics Engineers Inc., 2022) Al-Maghrabi, Latifah; Martins, Patricia; Silva, Daniela; Gil, Guilherme; Barroca, Nathalie; Murua, Olatz; Olalde, Beatriz; Alves, Luis; Pedreiras, Paulo; Fonseca, Pedro; Leduc, Philip; Marques, Paula; SG; BiomaterialesCircuit modeling of scaffolds based on porcine adipose decellularized extracellular matrix (adECM) doped with reduced graphene oxide (rGO) for spinal cord regeneration is presented. The characteristics of the scaffolds capped with silver electrodes were studied in an aqueous medium through transient and AC analyses. In addition, cyclic voltammetry (CV) plots were obtained. The transient measurements were done using a custom current driver while the CV and AC measurements were obtained with an external impedance analyzer. The results revealed that incorporating rGO reduced the series resistance and the impedance at low frequencies of the scaffold.Item Interfacing reduced graphene oxide with an adipose-derived extracellular matrix as a regulating milieu for neural tissue engineering(2023-05) Barroca, Nathalie; da Silva, Daniela M.; Pinto, Susana C.; Sousa, Joana P.M.; Verstappen, Kest; Klymov, Alexey; Fernández-San-Argimiro, Francisco Javier; Madarieta, Iratxe; Murua, Olatz; Olalde, Beatriz; Papadimitriou, Lina; Karali, Kanelina; Mylonaki, Konstantina; Stratakis, Emmanuel; Ranella, Anthi; Marques, Paula A.A.P.; Biomateriales; SGEnthralling evidence of the potential of graphene-based materials for neural tissue engineering is motivating the development of scaffolds using various structures related to graphene such as graphene oxide (GO) or its reduced form. Here, we investigated a strategy based on reduced graphene oxide (rGO) combined with a decellularized extracellular matrix from adipose tissue (adECM), which is still unexplored for neural repair and regeneration. Scaffolds containing up to 50 wt% rGO relative to adECM were prepared by thermally induced phase separation assisted by carbodiimide (EDC) crosslinking. Using partially reduced GO enables fine-tuning of the structural interaction between rGO and adECM. As the concentration of rGO increased, non-covalent bonding gradually prevailed over EDC-induced covalent conjugation with the adECM. Edge-to-edge aggregation of rGO favours adECM to act as a biomolecular physical crosslinker to rGO, leading to the softening of the scaffolds. The unique biochemistry of adECM allows neural stem cells to adhere and grow. Importantly, high rGO concentrations directly control cell fate by inducing the differentiation of both NE-4C cells and embryonic neural progenitor cells into neurons. Furthermore, primary astrocyte fate is also modulated as increasing rGO boosts the expression of reactivity markers while unaltering the expression of scar-forming ones.