TY  - Journal Article
AU  - Cicuéndez, Mónica
AU  - Casarrubios, Laura
AU  - Feito, María José
AU  - Madarieta, Iratxe
AU  - Garcia-Urkia, Nerea
AU  - Murua, Olatz
AU  - Olalde, Beatriz
AU  - Briz, Nerea
AU  - Diez-Orejas, Rosalía
AU  - Portolés, María Teresa
TI  - Candida albicans/Macrophage Biointerface on Human and Porcine Decellularized Adipose Matrices
PY  - 2021
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
AB  - Macrophages, cells effective in sensing, internalizing and killing Candida albicans, are intertwined
with the extracellular matrix (ECM) through different signals, which include the release of specific
cytokines. Due to the importance of these interactions, the employment of in vitro models mimicking
a fungal infection scenario is essential to evaluate the ECM effects on the macrophage response.
In this work, we have analyzed the effects of human and porcine decellularized adipose matrices
(DAMs), obtained by either enzymatic or organic solvent treatment, on the macrophage/Candida albicans
interface. The present study has allowed us to detect differences on the activation of macrophages
cultured on either human- or porcine-derived DAMs, evidencing changes in the macrophage actin
cytoskeleton, such as distinct F-actin-rich membrane structures to surround the pathogen. The
macrophage morphological changes observed on these four DAMs are key to understand the defense
capability of these cells against this fungal pathogen. This work has contributed to the knowledge of
the influence that the extracellular matrix and its components can exert on macrophage metabolism,
immunocompetence and capacity to respond to the microenvironment in a possible infection scenario.
UR  - http://hdl.handle.net/11556/1148
DX  - 10.3390/jof7050392
ER  -