Show simple item record

dc.contributor.authorMantecón-Oria, Marián
dc.contributor.authorDiban, Nazely
dc.contributor.authorBerciano, Maria T.
dc.contributor.authorRivero, Maria J.
dc.contributor.authorDavid, Oana
dc.contributor.authorLafarga, Miguel
dc.contributor.authorTapia, Olga
dc.contributor.authorUrtiaga, Ane
dc.date.accessioned2020-09-11T10:14:10Z
dc.date.available2020-09-11T10:14:10Z
dc.date.issued2020-08
dc.identifier.citationMantecón-Oria, Marián, Nazely Diban, Maria T. Berciano, Maria J. Rivero, Oana David, Miguel Lafarga, Olga Tapia, and Ane Urtiaga. “Hollow Fiber Membranes of PCL and PCL/Graphene as Scaffolds with Potential to Develop In Vitro Blood—Brain Barrier Models.” Membranes 10, no. 8 (July 22, 2020): 161. doi:10.3390/membranes10080161.en
dc.identifier.urihttp://hdl.handle.net/11556/976
dc.description.abstractThere is a huge interest in developing novel hollow fiber (HF) membranes able to modulate neural differentiation to produce in vitro blood–brain barrier (BBB) models for biomedical and pharmaceutical research, due to the low cell-inductive properties of the polymer HFs used in current BBB models. In this work, poly(ε-caprolactone) (PCL) and composite PCL/graphene (PCL/G) HF membranes were prepared by phase inversion and were characterized in terms of mechanical, electrical, morphological, chemical, and mass transport properties. The presence of graphene in PCL/G membranes enlarged the pore size and the water flux and presented significantly higher electrical conductivity than PCL HFs. A biocompatibility assay showed that PCL/G HFs significantly increased C6 cells adhesion and differentiation towards astrocytes, which may be attributed to their higher electrical conductivity in comparison to PCL HFs. On the other hand, PCL/G membranes produced a cytotoxic effect on the endothelial cell line HUVEC presumably related with a higher production of intracellular reactive oxygen species induced by the nanomaterial in this particular cell line. These results prove the potential of PCL HF membranes to grow endothelial cells and PCL/G HF membranes to differentiate astrocytes, the two characteristic cell types that could develop in vitro BBB models in future 3D co-culture systems.en
dc.description.sponsorshipThis research was funded by IDIVAL (INNVAL 17/20), MINECO/EIG-Concert Japan (X-MEM PCI2018-092929 project, International Joint Program 2018) and MINECO/Spain Feder (CTM-2016-75509-R project).en
dc.language.isoengen
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI)en
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleHollow Fiber Membranes of PCL and PCL/Graphene as Scaffolds with Potential to Develop In Vitro Blood—Brain Barrier Modelsen
dc.typearticleen
dc.identifier.doi10.3390/membranes10080161en
dc.rights.accessRightsopenAccessen
dc.subject.keywordsMixed-matrix hollow fibersen
dc.subject.keywordsGrapheneen
dc.subject.keywordsPoly(ε-caprolactone)en
dc.subject.keywords3D cell culturesen
dc.subject.keywordsIn vitro blood brain barrier (BBB) modelen
dc.identifier.essn2077-0375en
dc.issue.number8en
dc.journal.titleMembranesen
dc.page.initial161en
dc.volume.number10en


Files in this item

Thumbnail

    Show simple item record

    Attribution 4.0 InternationalExcept where otherwise noted, this item's license is described as Attribution 4.0 International