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dc.contributor.authorBidaguren, Aritz
dc.contributor.authorMendicute, Javier
dc.contributor.authorMadarieta, Iratxe
dc.contributor.authorGaragorri, Nerea
dc.date.accessioned2020-02-04T16:01:22Z
dc.date.available2020-02-04T16:01:22Z
dc.date.issued2019-11
dc.identifier.citationBidaguren, Aritz, Javier Mendicute, Iratxe Madarieta, and Nerea Garagorri. “Confocal and Histological Features After Poly(Ethylene Glycol) Diacrylate Corneal Inlay Implantation.” Translational Vision Science & Technology 8, no. 6 (December 18, 2019): 39. doi:10.1167/tvst.8.6.39.en
dc.identifier.urihttp://hdl.handle.net/11556/868
dc.description.abstractPurpose: To evaluate the in vivo biocompatibility of photopolymerized poly(ethylene glycol) diacrylate (PEGDA) intrastromal inlays in rabbit corneas. Methods: Sixty-three eyes of 42 New Zealand rabbits were included. Manual intrastromal pockets were dissected in 42 eyes. PEGDA inlays were obtained using a specifically designed photomask and were inserted in the intrastromal pocket of 21 eyes (inlay group); the remaining 21 right eyes did not receive any implant (pocket-only group). Twenty-one eyes with no intervention were used as controls. In vivo confocal microscopy (IVCM) was performed at every visit. After 2 months, rabbits were sacrificed and corneas removed for histological analysis. Results: Corneas remained clear in all but two animals, and five cases of corneal neovascularization were seen (P = 0.2). Inlays remained stable without evidence of lateral or anterior migration, and no other complications were observed. No changes in anterior and posterior keratocyte density (P = 0.3 and P = 0.1, respectively) or endothelial cell density (P = 0.23) were observed between groups during the study time by IVCM. On pathology samples, thinning of the epithelium over the inlay area and epithelial hyperplasia over the edges were observed. A polygonal empty space with no evidence of PEGDA hydrogel within the midstroma was seen in the inlay group. Keratocytes were normal in shape and number in the vicinity of the PEGDA implant area. Conclusions: Photopolymerized PEGDA intrastromal inlays have shown relatively good safety and stability in rabbit corneas. Inlays were biostable in the corneal environment and remained transparent during follow up. Translational Relevance: The investigated PEGDA is promising for the development of biocompatible intrastromal implants.en
dc.description.sponsorshipSupported in part by the Spanish Ministry of Science and Education through Apply Research, grant MAT2006-13708-CO2-01. The authors alone are responsible for the content and writing of the paper. Disclosure: A. Bidaguren, None; J. Mendicute, None; I. Madarieta, Tecnalia (E); N. Garagorri, Tecnalia (E)en
dc.language.isoengen
dc.publisherAssociation for Research in Vision and Ophthalmology Inc.en
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleConfocal and Histological Features After Poly(Ethylene Glycol) Diacrylate Corneal Inlay Implantationen
dc.typearticleen
dc.identifier.doi10.1167/tvst.8.6.39en
dc.rights.accessRightsopenAccessen
dc.subject.keywordsConfocal microscopyen
dc.subject.keywordsPoly(ethylene glycol) diacrylateen
dc.subject.keywordsHydrogelen
dc.subject.keywordsIntracorneal inlayen
dc.subject.keywordsCorneal stromaen
dc.identifier.essn2164-2591en
dc.issue.number6en
dc.journal.titleTranslational Vision Science & Technologyen
dc.page.initial39en
dc.volume.number8en


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