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dc.contributor.authorHaas, C.
dc.contributor.authorSteinwandter, V.
dc.contributor.authorDiaz De Apodaca, E.
dc.contributor.authorMaestro Madurga, B.
dc.contributor.authorSmerilli, M.
dc.contributor.authorDietrich, T.
dc.contributor.authorNeureiter, M.
dc.date.accessioned2015-09-21T15:11:24Z
dc.date.available2015-09-21T15:11:24Z
dc.date.issued2015-06-03
dc.identifier.citationChem. Biochem. Eng. Q., Vol. 29, no. 2 (2015), pp. 99–112en
dc.identifier.issn1846-5153en
dc.identifier.urihttp://hdl.handle.net/11556/52
dc.description.abstractChicory roots from hydroponic salad cultivation are an abundant food residue in Navarra (Spain) that are underutilized to date. Aiming at a holistic utilization of resources, we report here the first process using chicory root hydrolysate for the production of poly([R]-3-hydroxybutyrate) (PHB). The polymer can be used for packaging material made for the locally produced vegetables. In the first step, we developed a pre-treatment process to obtain a hydrolysate, which contained 34 g L-1 sugars and 0.7 g L-1 total Kjeldahl nitrogen. This hydrolysate was used as fermentation substrate for three PHB-producing strains. Cupriavidus necator DSM 428 reached a dry biomass concentration of 11.3 g L-1 with a PHB content of 66 % in dry mass within 5 days. C. necator DSM 531 yielded 3.5 g L-1 dry biomass containing 46 % PHB within the same period. C. necator DSM 545 was superior over the other two in that 14.0 g L-1 of biomass containing 78 % PHB after only 3 days were obtained. These results show that even within the same species, the productivities on natural substrates are very different. The produced polymers were extracted using chloroform, and several thermo-physical parameters are in good accordance with published data. Overall, our holistic approach and the encouraging results prove that chicory roots are a viable fermentation substrate for PHB-production.en
dc.description.sponsorshipThis work was conducted as a part of the LEAD-ERA Project CARBIO, which was financed by the Basque Goverment and co-financed by the European Regional Development Fund (ERDF) of the European Union.en
dc.language.isoengen
dc.publisherCROATIAN SOC CHEMICAL ENGINEERING TECHNOLOGY, BERISLAVICEVA 6, PO BOX 123, HR-10000 ZAGREB, CROATIAen
dc.titleProduction of PHB from Chicory Roots - Comparison of Three Cupriavidus necator Strainsen
dc.typearticleen
dc.identifier.doi10.15255/CABEQ.2014.2250en
dc.isiYesen
dc.rights.accessRightsopenAccessen
dc.subject.keywordsCichorium intybusen
dc.subject.keywordsfructoseen
dc.subject.keywordsby-producten
dc.subject.keywordsfood residueen
dc.subject.keywordspre-treatmenten
dc.subject.keywordshydrolysateen
dc.subject.keywordsbatch fermentationen
dc.subject.keywordsCupriavidus necatoren
dc.subject.keywordsP3HB; polymer characterisationen
dc.subject.keywordsinulinen


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