Production of PHB from Chicory Roots - Comparison of Three Cupriavidus necator Strains
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Author/sHaas, C.; Steinwandter, V.; Diaz De Apodaca, E.; Maestro Madurga, B.; Smerilli, M.; [et al.]
P3HB; polymer characterisation
Chicory roots from hydroponic salad cultivation are an abundant food residue in Navarra (Spain) that are underutilized to date. Aiming at a holistic utilization of resources, we report here the first process using chicory root hydrolysate for the production of poly([R]-3-hydroxybutyrate) (PHB). The polymer can be used for packaging material made for the locally produced vegetables. In the first step, we developed a pre-treatment process to obtain a hydrolysate, which contained 34 g L-1 sugars and 0.7 g L-1 total Kjeldahl nitrogen. This hydrolysate was used as fermentation substrate for three PHB-producing strains. Cupriavidus necator DSM 428 reached a dry biomass concentration of 11.3 g L-1 with a PHB content of 66 % in dry mass within 5 days. C. necator DSM 531 yielded 3.5 g L-1 dry biomass containing 46 % PHB within the same period. C. necator DSM 545 was superior over the other two in that 14.0 g L-1 of biomass containing 78 % PHB after only 3 days were obtained. These results ...