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dc.contributor.authorBraceras, I.
dc.contributor.authorOnate, J.I.
dc.contributor.authorGoikoetxea, L.
dc.contributor.authorViviente, J.L.
dc.contributor.authorAlava, J.I.
dc.contributor.authorde Maeztu, M.A.
dc.date.accessioned2016-09-26T08:48:27Z
dc.date.available2016-09-26T08:48:27Z
dc.date.issued2005-06-22
dc.identifier.citationSurface and Coatings Technology, Volume 196, Issues 1–3, 22 June 2005, Pages 321-326en
dc.identifier.issn0257-8972en
dc.identifier.urihttp://hdl.handle.net/11556/294
dc.description.abstractThe authors have previously reported than ion implantation can have a significant effect on osseointegration of an implant, specially when the latter is introduced in areas of poorer bone density. These results indicate that this process is particularly suited for implant devices introduced in elderly patients or in those regions that have a poor quality of bone. The aim of this work is directed to study osteoblast adhesion on Ti alloy surfaces with different ion implantation treatments, so osseoconductive properties of several surfaces can be assessed. Polished discs of Ti–6Al–4V and Ti CP GR1 titanium alloy have been prepared and ion implanted with different species and parameters (dose and energy). Afterwards, the samples have been sterilized by UV light, inoculated with 1.5×105 human bone cells and incubated during 4 h at 37 C and 5% CO2 atmosphere. Then, once fixed and rinsed, image analysis has been used to quantify the number of cells attached to the Ti discs. On a second round of tests, cell proliferation tests have been conducted during 24, 48, 144 and 192 h, respectively. Furthermore, surface analysis techniques (e.g. AFM) have been applied to learn about the qualitative behavior, i.e. morphology, of the attached cells. Cell attachment has shown to be highly sensitive to ion implantation parameters. Although some quantitative differences have been observed, the more significant differences were qualitative. AFM analysis has shown that the star-shaped bone cells attached spread more and occupied larger surfaces like in osseointegration prone surfaces, most probably due to extracellular matrix synthesized around them, while other surfaces showed mainly large and narrow shaped or round shaped bone cells often with great cellular nucleus in the middle of the cells and little extracellular matrix around. So, ion implanted surfaces that facilitate osseointegration have been identified, in terms of initial bone cell attachment quality, where although the number of attached cells were not necessarily always larger, they tended to occupy wider areas with healthier cells.en
dc.description.sponsorshipBasque Government (ETORTEK MAAB); Spanish Ministry of Science of Technology-CDTI (Neotec Ref. 20020327)en
dc.language.isoengen
dc.publisherElsevieren
dc.titleBone cell adhesion on ion implanted titanium alloysen
dc.typeconferenceObjecten
dc.identifier.doi10.1016/j.surfcoat.2004.08.201en
dc.isiYesen
dc.rights.accessRightsembargoedAccessen
dc.subject.keywordsIon implantationen
dc.subject.keywordsTitanium alloysen
dc.subject.keywordsOsteoblasten
dc.subject.keywordsCell cultureen
dc.subject.keywordsImplanten
dc.subject.keywordsCell proliferationen


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