Show simple item record

dc.contributor.authordel Barrio, J.L.A.
dc.contributor.authorChiesa, M.
dc.contributor.authorGallego Ferrer, Gloria
dc.contributor.authorGaragorri, N.
dc.contributor.authorBriz, N.
dc.contributor.authorFernandez-Delgado, Jorge
dc.contributor.authorSancho-Tello Valls, Maria
dc.contributor.authorCarda Botella, Carmen
dc.contributor.authorGarcia-Tunon, Ignacio
dc.contributor.authorBataille, Laurent
dc.contributor.authorRodriguez, Alejandra
dc.contributor.authorArnalich-Montiel, Francisco
dc.contributor.authorGomez Ribelles, Jose L.
dc.contributor.authorAntolinos-Turpin, Carmen M.
dc.contributor.authorGomez-Tejedor, Jose A.
dc.contributor.authorAlio, Jorge L.
dc.contributor.authorDe Miguel, Maria P.
dc.date.accessioned2016-05-03T14:53:41Z
dc.date.available2016-05-03T14:53:41Z
dc.date.issued2015-03
dc.identifier.citationJOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, Vol. 103, Issue 3 (2015), pp. 1106-1118en
dc.identifier.issn1549-3296en
dc.identifier.urihttp://hdl.handle.net/11556/227
dc.description.abstractCurrently available keratoprosthesis models (nonbiological corneal substitutes) have a less than 75% graft survival rate at 2 years. We aimed at developing a model for keratoprosthesis based on the use of poly(ethyl acrylate) (PEA)-based copolymers, extracellular matrix-protein coating and colonization with adipose-derived mesenchymal stem cells. Human adipose tissue derived mesenchymal stem cells (h-ADASC) colonization efficiency of seven PEA-based copolymers in combination with four extracellular matrix coatings were evaluated in vitro. Then, macroporous membranes composed of the optimal PEA subtypes and coating proteins were implanted inside rabbit cornea. After a 3-month follow-up, the animals were euthanized, and the clinical and histological biointegration of the implanted material were assessed. h-ADASC adhered and survived when cultured in all PEA-based macroporous membranes. The addition of high hydrophilicity to PEA membranes decreased h-ADASC colonization in vitro. PEA-based copolymer containing 10% hydroxyethyl acrylate (PEA-HEA10) or 10% acrylic acid (PEA-AAc10) monomeric units showed the best cellular colonization rates. Collagen plus keratan sulfate-coated polymers demonstrated enhanced cellular colonization respect to fibronectin, collagen, or uncoated PEAs. In vivo implantation of membranes resulted in an extrusion rate of 72% for PEA, 50% for PEA-AAc10, but remarkably of 0% for PEA-HEA10. h-ADASC survival was demonstrated in all the membranes after 3 months follow-up. A slight reduction in the extrusion rate of h-ADASC colonized materials was observed. No significant differences between the groups with and without h-ADASC were detected respect to transparency or neovascularization. We propose PEA with low hydroxylation as a scaffold for the anchoring ring of future keratoprosthesis.en
dc.description.sponsorshipSpanish Ministry of Health Spanish Ministry of Science and Innovation Fundacio Marato de TV3, Spainen
dc.language.isoengen
dc.publisherWILEY-BLACKWELL, 111 RIVER ST, HOBOKEN 07030-5774, NJ USAen
dc.titleBiointegration of corneal macroporous membranes based on poly(ethyl acrylate) copolymers in an experimental animal modelen
dc.typearticleen
dc.identifier.doi10.1002/jbm.a.35249en
dc.isiYesen
dc.rights.accessRightsembargoedAccessen
dc.subject.keywordspoly(ethyl acrylate)en
dc.subject.keywordsadipose derived stem cellsen
dc.subject.keywordscorneaen
dc.subject.keywordsmacroporous membranesen
dc.subject.keywordscorneal scaffolden
dc.subject.keywordsMESENCHYMAL STEM-CELLSen
dc.subject.keywordsHUMAN ADIPOSE-TISSUEen
dc.subject.keywordsKERATOPROSTHESISen
dc.subject.keywordsCOLLAGENen
dc.subject.keywordsFIBRONECTINen
dc.subject.keywordsSCAFFOLDSen
dc.subject.keywordsIMPLANTen


Files in this item

FilesSizeFormatView

There are no files associated with this item.

    Show simple item record