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dc.contributor.authordel Barrio, J.L.A.
dc.contributor.authorChiesa, M.
dc.contributor.authorGaragorri, N.
dc.contributor.authorGarcia-Urquia, Nerea
dc.contributor.authorFernandez-Delgado, Jorge
dc.contributor.authorBataille, Laurent
dc.contributor.authorRodriguez, Alejandra
dc.contributor.authorArnalich-Montiel, Francisco
dc.contributor.authorZarnowski, Tomasz
dc.contributor.authorAlvarez de Toledo, Juan P.
dc.contributor.authorAlio, Jorge L.
dc.contributor.authorDe Miguel, Maria P.
dc.date.accessioned2016-04-12T13:54:31Z
dc.date.available2016-04-12T13:54:31Z
dc.date.issued2015-03
dc.identifier.citationEXPERIMENTAL EYE RESEARCH, 132 (2015), pp. 91-100en
dc.identifier.issn1096-0007en
dc.identifier.urihttp://hdl.handle.net/11556/186
dc.description.abstractPurpose: To evaluate the in vivo biocompatibility of grafts composed of sheets of decellularized human corneal stroma with or without the recellularization of human adipose derived adult stem cells (h-ADASC) into the rabbit cornea. Methods: Sheets of human corneal stroma of 90 gm thickness were decellularized, and their lack of cytotoxicity was assayed. The recellularization was achieved by the injection of 2 x 10(5) labeled h-ADASC in the graft followed by five days of cell culture. The grafts were implanted in vivo into a stromal pocket at 50% depth. After a triple-masked three-month follow-up, the animals were euthanized and the bio-integration of the graft, the viability of the stem cells and the expression of keratocan (human keratocyte-specific protein) were assessed. Results: The decellularized stromal sheets showed an intact extracellular matrix with a decellularization rate of 92.8% and an excellent recellularization capacity in vitro with h-ADASC. A complete and stable graft transparency was observed during the full follow-up, with absence of any clinical sign of rejection. The postmortem analysis demonstrated the survival of the transplanted human stem cells inside the graft and their differentiation into functional keratocytes, as assessed by the expression of human keratocan. Conclusions: We report a new model of lamellar keratoplasty that requires only a simple and safe procedure of liposuction and a donor allogeneic cornea to provide an optically transparent autologous stromal graft with excellent biocompatibility and integration into the host tissue in a rabbit modelen
dc.description.sponsorshipThis work was supported in part by grants CEN-20091021 from the Spanish Ministry of Health, IAP-560610-2008-44 and SAF2010-19230 from the Spanish Ministry of Science and Innovation, and from Fundacio Marato de TV3, Spain.en
dc.language.isoengen
dc.publisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD, 24-28 OVAL RD, LONDON NW1 7DX, ENGLANDen
dc.titleAcellular human corneal matrix sheets seeded with human adipose-derived mesenchymal stem cells integrate functionally in an experimental animal modelen
dc.typearticleen
dc.identifier.doi10.1016/j.exer.2015.01.020en
dc.isiYesen
dc.rights.accessRightsembargoedAccessen
dc.subject.keywordsAdipose derived stem cellsen
dc.subject.keywordsCorneaen
dc.subject.keywordsDecellularizationen
dc.subject.keywordsRecellularizationen
dc.subject.keywordsLamellar corneal transplanten
dc.subject.keywordsCorneal stroma regenerationen
dc.subject.keywordsTissue engineeringen


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