Acellular human corneal matrix sheets seeded with human adipose-derived mesenchymal stem cells integrate functionally in an experimental animal model
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Author/sdel Barrio, J.L.A.; Chiesa, M.; Garagorri, N.; Garcia-Urquia, Nerea; Fernandez-Delgado, Jorge; [et al.]
Adipose derived stem cells
Lamellar corneal transplant
Corneal stroma regeneration
Purpose: To evaluate the in vivo biocompatibility of grafts composed of sheets of decellularized human corneal stroma with or without the recellularization of human adipose derived adult stem cells (h-ADASC) into the rabbit cornea. Methods: Sheets of human corneal stroma of 90 gm thickness were decellularized, and their lack of cytotoxicity was assayed. The recellularization was achieved by the injection of 2 x 10(5) labeled h-ADASC in the graft followed by five days of cell culture. The grafts were implanted in vivo into a stromal pocket at 50% depth. After a triple-masked three-month follow-up, the animals were euthanized and the bio-integration of the graft, the viability of the stem cells and the expression of keratocan (human keratocyte-specific protein) were assessed. Results: The decellularized stromal sheets showed an intact extracellular matrix with a decellularization rate of 92.8% and an excellent recellularization capacity in vitro with h-ADASC. A complete and stable graft ...