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dc.contributor.authorLuzuriaga, J
dc.contributor.authorGarcía-Gallastegui, P
dc.contributor.authorGarcía-Urkia, N
dc.contributor.authorPineda, JR
dc.contributor.authorIrastorza, I
dc.contributor.authorFernandez-San-Argimiro, F-J
dc.contributor.authorOlalde, B
dc.contributor.authorUnda, F
dc.contributor.authorMadarieta, I
dc.contributor.authorIbarretxe, G
dc.contributor.authorBriz, Nerea
dc.date.accessioned2022-09-02T10:53:18Z
dc.date.available2022-09-02T10:53:18Z
dc.date.issued2022
dc.identifier.citationLuzuriaga, J, P García-Gallastegui, N García-Urkia, JR Pineda, I Irastorza, F-J Fernandez-San-Argimiro, et al. “Osteogenic Differentiation of Human Dental Pulp Stem Cells in Decellularised Adipose Tissue Solid Foams.” European Cells and Materials 43 (March 21, 2022): 112–129. doi:10.22203/ecm.v043a10.en
dc.identifier.issn1473-2262en
dc.identifier.urihttp://hdl.handle.net/11556/1400
dc.description.abstract3D cell culture systems based on biological scaffold materials obtainable from both animal and human tissues constitute very interesting tools for cell therapy and personalised medicine applications. The white adipose tissue (AT) extracellular matrix (ECM) is a very promising biomaterial for tissue engineering due to its easy accessibility, malleability and proven biological activity. In the present study, human dental pulp stem cells (hDPSCs) were combined in vitro with ECM scaffolds from porcine and human decellularised adipose tissues (pDAT, hDAT) processed as 3D solid foams, to investigate their effects on the osteogenic differentiation capacity and bone matrix production of hDPSCs, compared to single-protein-based 3D solid foams of collagen type I and conventional 2D tissue-culture-treated polystyrene plates. pDAT solid foams supported the osteogenic differentiation of hDPSCs to similar levels to collagen type I, as assessed by alkaline phosphatase and alizarin red stainings, reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) and osteocalcin/bone gamma-carboxyglutamate protein (BGLAP) immunostaining. Interestingly, hDAT solid foams showed a markedly lower capacity to sustain hDPSC osteogenic differentiation and matrix calcification and a higher capacity to support adipogenesis, as assessed by RT-qPCR and oil red O staining. White ATs from both human and porcine origins are relatively abundant and available sources of raw material to obtain high quality ECM-derived biomedical products. These biomaterials could have promising applications in tissue engineering and personalised clinical therapy for the healing and regeneration of lesions involving not only a loss of calcified bone but also its associated soft non-calcified tissues.en
dc.description.sponsorshipThis research was supported by the Basque Government (ELKARTEK program PLAKA KK2019-00093; to NB), MICINN retos I+D+i (PID2019- 104766RB-C21, to JRP) and UPV/EHU (PPGA20/22; to FU, GI). The authors would like to thank the staff members of the SGIKER services of the UPV/EHU: Lipidomic service (Beatriz Abad) and Analytical Microscopy (Ricardo Andrade, Alejandro Díez-Torre and Irene Fernández) for their technical assistance.en
dc.language.isoengen
dc.publisherAO Research Institute Davosen
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleOsteogenic differentiation of human dental pulp stem cells in decellularised adipose tissue solid foamsen
dc.typejournal articleen
dc.identifier.doi10.22203/ecm.v043a10en
dc.rights.accessRightsopen accessen
dc.subject.keywordsDental pulp stem cellsen
dc.subject.keywordsAdipose tissueen
dc.subject.keywordsBone tissueen
dc.subject.keywordsExtracellular matrixen
dc.subject.keywordsDecellularisationen
dc.subject.keywordsSolid foamen
dc.subject.keywordsOsteogenic differentiationen
dc.subject.keywordsMineralisationen
dc.journal.titleEuropean Cells and Materialsen
dc.page.final129en
dc.page.initial112en
dc.volume.number43en


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