Candida albicans/Macrophage Biointerface on Human and Porcine Decellularized Adipose Matrices
Author/s
Cicuéndez, Mónica; Casarrubios, Laura; Feito, María José; Madarieta, Iratxe; Garcia-Urkia, Nerea; [et al.]Date
2021-05-17Keywords
Candida albicans
Macrophage
Extracellular matrix
Decellularization
Immunocompetence
Phagocytosis
Abstract
Macrophages, cells effective in sensing, internalizing and killing Candida albicans, are intertwined
with the extracellular matrix (ECM) through different signals, which include the release of specific
cytokines. Due to the importance of these interactions, the employment of in vitro models mimicking
a fungal infection scenario is essential to evaluate the ECM effects on the macrophage response.
In this work, we have analyzed the effects of human and porcine decellularized adipose matrices
(DAMs), obtained by either enzymatic or organic solvent treatment, on the macrophage/Candida albicans
interface. The present study has allowed us to detect differences on the activation of macrophages
cultured on either human- or porcine-derived DAMs, evidencing changes in the macrophage actin
cytoskeleton, such as distinct F-actin-rich membrane structures to surround the pathogen. The
macrophage morphological changes observed on these four DAMs are key to understand the defense
capability ...
Type
journal article