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dc.contributor.authorCicuéndez, Mónica
dc.contributor.authorCasarrubios, Laura
dc.contributor.authorFeito, María José
dc.contributor.authorMadarieta, Iratxe
dc.contributor.authorGarcia-Urkia, Nerea
dc.contributor.authorMurua, Olatz
dc.contributor.authorOlalde, Beatriz
dc.contributor.authorBriz, Nerea
dc.contributor.authorDiez-Orejas, Rosalía
dc.contributor.authorPortolés, María Teresa
dc.date.accessioned2021-04-19T09:02:11Z
dc.date.available2021-04-19T09:02:11Z
dc.date.issued2021-04-08
dc.identifier.citationCicuéndez, Mónica, Laura Casarrubios, María José Feito, Iratxe Madarieta, Nerea Garcia-Urkia, Olatz Murua, Beatriz Olalde, Nerea Briz, Rosalía Diez-Orejas, and María Teresa Portolés. “Effects of Human and Porcine Adipose Extracellular Matrices Decellularized by Enzymatic or Chemical Methods on Macrophage Polarization and Immunocompetence.” International Journal of Molecular Sciences 22, no. 8 (April 8, 2021): 3847. doi:10.3390/ijms22083847.en
dc.identifier.issn16616596en
dc.identifier.urihttp://hdl.handle.net/11556/1115
dc.description.abstractThe decellularized extracellular matrix (ECM) obtained from human and porcine adipose tissue (AT) is currently used to prepare regenerative medicine bio-scaffolds. However, the influence of these natural biomaterials on host immune response is not yet deeply understood. Since macrophages play a key role in the inflammation/healing processes due to their high functional plasticity between M1 and M2 phenotypes, the evaluation of their response to decellularized ECM is mandatory. It is also necessary to analyze the immunocompetence of macrophages after contact with decellularized ECM materials to assess their functional role in a possible infection scenario. In this work, we studied the effect of four decellularized adipose matrices (DAMs) obtained from human and porcine AT by enzymatic or chemical methods on macrophage phenotypes and fungal phagocytosis. First, a thorough biochemical characterization of these biomaterials by quantification of remnant DNA, lipids, and proteins was performed, thus indicating the efficiency and reliability of both methods. The proteomic analysis evidenced that some proteins are differentially preserved depending on both the AT origin and the decellularization method employed. After exposure to the four DAMs, specific markers of M1 proinflammatory and M2 anti-inflammatory macrophages were analyzed. Porcine DAMs favor the M2 phenotype, independently of the decellularization method employed. Finally, a sensitive fungal phagocytosis assay allowed us to relate the macrophage phagocytosis capability with specific proteins differentially preserved in certain DAMs. The results obtained in this study highlight the close relationship between the ECM biochemical composition and the macrophage’s functional role.en
dc.description.sponsorshipThis work has been supported by the European Union’s Horizon 2020 Research and Innovation Programme (H2020-FETOPEN-2018-2020, NeuroStimSpinal Project, Grant Agreement No. 829060). M.C. acknowledges the European Union0s Horizon 2020 Research and Innovation Programme for her contract under the NeuroStimSpinal Project. LC is grateful to the Universidad Complutense de Madrid for an UCM fellowship.en
dc.language.isoengen
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI)
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleEffects of Human and Porcine Adipose Extracellular Matrices Decellularized by Enzymatic or Chemical Methods on Macrophage Polarization and Immunocompetenceen
dc.typearticleen
dc.identifier.doi10.3390/ijms22083847en
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/H2020/829060/EU/A STEP FORWARD TO SPINAL CORD INJURY REPAIR USING INNOVATIVE STIMULATED NANOENGINEERED SCAFFOLDS/NeuroStimSpinalen
dc.rights.accessRightsopenAccessen
dc.subject.keywordsExtracellular matrixen
dc.subject.keywordsDecellularizationen
dc.subject.keywordsMacrophageen
dc.subject.keywordsImmunocompetenceen
dc.subject.keywordsPhagocytosisen
dc.identifier.essn1422-0067en
dc.issue.number8en
dc.journal.titleInternational Journal of Molecular Sciencesen
dc.page.initial3847en
dc.volume.number22en
dc.conference.title


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    Attribution 4.0 InternationalExcept where otherwise noted, this item's license is described as Attribution 4.0 International