dc.contributor.author | Bordanaba-Florit, Guillermo | |
dc.contributor.author | Madarieta, Iratxe | |
dc.contributor.author | Olalde, Beatriz | |
dc.contributor.author | Falcón-Pérez, Juan M. | |
dc.contributor.author | Royo, Félix | |
dc.date.accessioned | 2021-02-08T18:14:00Z | |
dc.date.available | 2021-02-08T18:14:00Z | |
dc.date.issued | 2021-01-15 | |
dc.identifier.citation | Bordanaba-Florit, Guillermo, Iratxe Madarieta, Beatriz Olalde, Juan M. Falcón-Pérez, and Félix Royo. “3D Cell Cultures as Prospective Models to Study Extracellular Vesicles in Cancer.” Cancers 13, no. 2 (January 15, 2021): 307. doi:10.3390/cancers13020307. | en |
dc.identifier.uri | http://hdl.handle.net/11556/1075 | |
dc.description.abstract | The improvement of culturing techniques to model the environment and physiological
conditions surrounding tumors has also been applied to the study of extracellular vesicles (EVs)
in cancer research. EVs role is not only limited to cell-to-cell communication in tumor physiology,
they are also a promising source of biomarkers, and a tool to deliver drugs and induce antitumoral
activity. In the present review, we have addressed the improvements achieved by using 3D culture
models to evaluate the role of EVs in tumor progression and the potential applications of EVs in
diagnostics and therapeutics. The most employed assays are gel-based spheroids, often utilized to
examine the cell invasion rate and angiogenesis markers upon EVs treatment. To study EVs as drug
carriers, a more complex multicellular cultures and organoids from cancer stem cell populations
have been developed. Such strategies provide a closer response to in vivo physiology observed
responses. They are also the best models to understand the complex interactions between different
populations of cells and the extracellular matrix, in which tumor-derived EVs modify epithelial or
mesenchymal cells to become protumor agents. Finally, the growth of cells in 3D bioreactor-like
systems is appointed as the best approach to industrial EVs production, a necessary step toward
clinical translation of EVs-based therapy. | en |
dc.description.sponsorship | The review is supported by Spanish Ministry of Science and Innovation, within the national
Plan RTI2018-094969-B-I00, and Excellence Severo Ochoa grant Innovative Research Grant (SEV-2016-
0644), and by the European Union’s Horizon 2020 research and innovation program, grant number
860303. | en |
dc.language.iso | eng | en |
dc.publisher | Multidisciplinary Digital Publishing Institute (MDPI) | en |
dc.rights | Attribution 4.0 International | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.title | 3D Cell Cultures as Prospective Models to Study Extracellular Vesicles in Cancer | en |
dc.type | journal article | en |
dc.identifier.doi | 10.3390/cancers13020307 | en |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/H2020/860303/EU/proEVLifeCycle | en |
dc.rights.accessRights | open access | en |
dc.subject.keywords | 3D culture | en |
dc.subject.keywords | extracellular vesicles | en |
dc.subject.keywords | tumoral cells | en |
dc.subject.keywords | cancer | en |
dc.subject.keywords | therapy | en |
dc.identifier.essn | 2072-6694 | en |
dc.issue.number | 2 | en |
dc.journal.title | Cancers | en |
dc.page.initial | 307 | en |
dc.volume.number | 13 | en |