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dc.contributor.authorBordanaba-Florit, Guillermo
dc.contributor.authorMadarieta, Iratxe
dc.contributor.authorOlalde, Beatriz
dc.contributor.authorFalcón-Pérez, Juan M.
dc.contributor.authorRoyo, Félix
dc.date.accessioned2021-02-08T18:14:00Z
dc.date.available2021-02-08T18:14:00Z
dc.date.issued2021-01-15
dc.identifier.citationBordanaba-Florit, Guillermo, Iratxe Madarieta, Beatriz Olalde, Juan M. Falcón-Pérez, and Félix Royo. “3D Cell Cultures as Prospective Models to Study Extracellular Vesicles in Cancer.” Cancers 13, no. 2 (January 15, 2021): 307. doi:10.3390/cancers13020307.en
dc.identifier.urihttp://hdl.handle.net/11556/1075
dc.description.abstractThe improvement of culturing techniques to model the environment and physiological conditions surrounding tumors has also been applied to the study of extracellular vesicles (EVs) in cancer research. EVs role is not only limited to cell-to-cell communication in tumor physiology, they are also a promising source of biomarkers, and a tool to deliver drugs and induce antitumoral activity. In the present review, we have addressed the improvements achieved by using 3D culture models to evaluate the role of EVs in tumor progression and the potential applications of EVs in diagnostics and therapeutics. The most employed assays are gel-based spheroids, often utilized to examine the cell invasion rate and angiogenesis markers upon EVs treatment. To study EVs as drug carriers, a more complex multicellular cultures and organoids from cancer stem cell populations have been developed. Such strategies provide a closer response to in vivo physiology observed responses. They are also the best models to understand the complex interactions between different populations of cells and the extracellular matrix, in which tumor-derived EVs modify epithelial or mesenchymal cells to become protumor agents. Finally, the growth of cells in 3D bioreactor-like systems is appointed as the best approach to industrial EVs production, a necessary step toward clinical translation of EVs-based therapy.en
dc.description.sponsorshipThe review is supported by Spanish Ministry of Science and Innovation, within the national Plan RTI2018-094969-B-I00, and Excellence Severo Ochoa grant Innovative Research Grant (SEV-2016- 0644), and by the European Union’s Horizon 2020 research and innovation program, grant number 860303.en
dc.language.isoengen
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI)en
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.title3D Cell Cultures as Prospective Models to Study Extracellular Vesicles in Canceren
dc.typearticleen
dc.identifier.doi10.3390/cancers13020307en
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/H2020/860303/EU/proEVLifeCycleen
dc.rights.accessRightsopenAccessen
dc.subject.keywords3D cultureen
dc.subject.keywordsextracellular vesiclesen
dc.subject.keywordstumoral cellsen
dc.subject.keywordscanceren
dc.subject.keywordstherapyen
dc.identifier.essn2072-6694en
dc.issue.number2en
dc.journal.titleCancersen
dc.page.initial307en
dc.volume.number13en


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