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dc.contributor.authorMartínez-Pérez, Paula
dc.contributor.authorPonce-Alcántara, Salvador
dc.contributor.authorMurillo, Nieves
dc.contributor.authorPérez-Márquez, Ana
dc.contributor.authorMaudes, Jon
dc.contributor.authorPeraile, Inés
dc.contributor.authorGonzález-López, Laura
dc.contributor.authorGil-García, Matilde
dc.contributor.authorLorenzo-Lozano, Paloma
dc.contributor.authorGarcía-Rupérez, Jaime
dc.date.accessioned2020-12-10T09:04:07Z
dc.date.available2020-12-10T09:04:07Z
dc.date.issued2020-11-26
dc.identifier.citationMartínez-Pérez, Paula, Salvador Ponce-Alcántara, Nieves Murillo, Ana Pérez-Márquez, Jon Maudes, Inés Peraile, Laura González-López, Matilde Gil-García, Paloma Lorenzo-Lozano, and Jaime García-Rupérez. “Label-Free Optical Biosensing Using Low-Cost Electrospun Polymeric Nanofibers.” Chemosensors 8, no. 4 (November 26, 2020): 119. doi:10.3390/chemosensors8040119.en
dc.identifier.urihttp://hdl.handle.net/11556/1030
dc.description.abstractPolymeric nanofiber matrices are promising structures to develop biosensing devices due to their easy and affordable large-scale fabrication and their high surface-to-volume ratio. In this work, the suitability of a polyamide 6 nanofiber matrix for the development of a label-free and real-time Fabry–Pérot cavity-based optical biosensor was studied. For such aim, in-flow biofunctionalization of nanofibers with antibodies, bound through a protein A/G layer, and specific biodetection of 10 µg/mL bovine serum albumin (BSA) were carried out. Both processes were successfully monitored via reflectivity measurements in real-time without labels and their reproducibility was demonstrated when different polymeric nanofiber matrices from the same electrospinning batch were employed as transducers. These results demonstrate not only the suitability of correctly biofunctionalized polyamide 6 nanofiber matrices to be employed for real-time and label-free specific biodetection purposes, but also the potential of electrospinning technique to create affordable and easy-to-fabricate at large scale optical transducers with a reproducible performance.en
dc.description.sponsorshipThis research was supported by a co-financed action by the European Union through the operational program of the European Regional Development Fund (FEDER) of the Valencian Community 2014–2020, the Generalitat Valenciana through the PROMETEO project AVANTI/2019/123 and the grant PPC/2020/037, the Spanish government through the project TEC2015-63838-C3-OPTONANOSENS, Universitat Politècnica de València through grant PAID-01-17, and by the Basque government through the project µ4Industry, KK-2019/00101, from the ELKARTEK Program.en
dc.language.isoengen
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI)en
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleLabel-Free Optical Biosensing Using Low-Cost Electrospun Polymeric Nanofibersen
dc.typearticleen
dc.identifier.doi10.3390/chemosensors8040119en
dc.rights.accessRightsopenAccessen
dc.subject.keywordsNanofibersen
dc.subject.keywordsPorous materialsen
dc.subject.keywordsFabry–pérot cavityen
dc.subject.keywordsOptical biosensoren
dc.subject.keywordsLabel-free biosensingen
dc.identifier.essn2227-9040en
dc.issue.number4en
dc.journal.titleChemosensorsen
dc.page.initial119en
dc.volume.number8en


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